 |
 |
 |
 |
 |
 |
Sains Malaysiana 53(11)(2024): 3663-3672
http://doi.org/10.17576/jsm-2024-5311-10
Intestinal
Piezo1 Promotes Neuroinflammation to Facilitate Oligodendrocyte Ferroptosis
Post-Traumatic Brain Injury
(Usus Piezo1 Menggalakkan Keradangan Neuro untuk Memudahkan Oligodendrocyte Ferroptosis Kecederaan Otak Pasca Traumatik)
DING ZHI JUN1, WU LIANG1,
CHEN ZHENG XIONG1, ZENG RUI MAO1, SHANGYUAN WANG2 & TANG MING ZHANG2,*
1Department
of Neurosurgery Department, Ningde Municipal Hospital
of Ningde Normal University, Fujian, China
2Department
of Emergency, Xinhua Hospital Affiliated to Shanghai Jiao Tong University
School of Medicine, Shanghai, China
Diserahkan 20 Ogos 2024/Diterima 23
September 2024
Abstract
The
coexistence of traumatic brain injury (TBI) and cognitive impairment is
increasingly common in clinical practice, but current research on identifying
biomarkers for such conditions and precise intervention points is insufficient.
This study utilized bioinformatics analysis with paired samples to explore
potential causal links between inflammatory factors and TBI and cognitive
impairment. By constructing a TBI model with Piezo1 gene knockout, we assessed
the activation status of microglia in the brain, the differentiation process of
oligodendrocyte precursor cells. The study identified a series of inflammatory
factors significantly associated with TBI, including C-C motif chemokine 19,
C-X-C motif chemokine 5, and interleukin-5. Bioinformatics analysis showed
increased expression of CXCL10, CCL2, GNA15, NFKB1, and the top 5 key nodes
were identified using the Cytohubba plugin. The
experimental results indicated that the knockout of the Piezo1 gene
significantly reduced the infiltration of microglia and neuroinflammation in
the brain.
Keywords: Bioinformatics; CXCL10; neuroinflammation; traumatic brain injury
Abstrak
Kewujudan kecederaan otak traumatis (TBI) dan gangguan kognitif semakin menjadi kebiasaan dalam amalan klinikal, tetapi penyelidikan semasa mengenai pengenalpastian penanda biologi untuk keadaan sedemikian dan titik intervensi yang tepat adalah tidak mencukupi.
Kajian ini menggunakan analisis bioinformatik dan randomisasi Mendelian (MR) dengan sampel bersama untuk meneroka hubungan kausal berpotensi antara faktor inflamasi dengan TBI dan gangguan kognitif. Dengan membina model TBI dengan penutupan gen Piezo1, kami menilai status pengaktifan mikroglia dalam otak, proses pembezaan sel-sel pendahulu oligodendrosit. Kajian mengenal pasti siri faktor inflamasi yang signifikan berkaitan dengan TBI, termasuk kemokina motif C-C 19, kemokina motif C-X-C 5 dan interleukin-5. Analisis bioinformatik menunjukkan peningkatan ekspresi CXCL10,
CCL2, GNA15, NFKB1 dan 5 nod kunci teratas dikenal pasti menggunakan plugin Cytohubba. Keputusan uji kaji menunjukkan bahawa penutupan gen Piezo1 secara signifikan mengurangkan infiltrasi mikroglia dalam otak dan melindungi pembezaan oligodendrosit.
Kata kunci: Bioinformatik;
CXCL10; kecederaan otak traumatis; keradangan neuro
RUJUKAN
Chen, M., Wang, H., Chen, P., Zhu, G., Li, S., Li,
Z., Liu, X., Ye, G. & Chen, W. 2024a. Neonatal microglia transplantation at
early stage but not late stage after traumatic brain injury shows protective
effects in mice. J. Neurophysiol. 131(4): 598-606.
doi:10.1152/jn.00006.2024
Chen, X., Ren, Y., Xie,
P., Lei, Q. & Lu, W. 2024b. GM130-silencing may aggravate blood-brain
barrier damage and affect microglia polarization by down-regulating PD-L1
expression after experimental intracerebral hemorrhage. Mol. Biol. Rep. 51(1):
919. doi:10.1007/s11033-024-09859-x
Czerpaniak, K., do Nascimento, L.F., Guo, T., Zhang, J., Liu,
X., Sarzaeim, M., Fine, Z.D. & Cao, Y.Q. 2024.
Low-dose interleukin-2 reverses traumatic brain injury-induced cognitive
deficit and pain in a murine model. Ann.
Neurol. 96(3): 508-525.
doi:10.1002/ana.26998
Dvorak, P., Hlavac, V., Hanicinec, V., Rao, B.H. & Soucek,
P. 2024. Genes divided according to the relative position of the longest intron
show increased representation in different KEGG pathways. BMC Genomics 25:
649. doi:10.1186/s12864-024-10558-x
Fujisawa, H., Watanabe, T., Komine,
O., Fuse, S., Masaki, M., Iwata, N., Murao, N.,
Seino, Y., Takeuchi, H, Yamanaka, K., Sawada, M., Suzuki, A. & Sugimura, Y.
2024. Prolonged extracellular low sodium concentrations and subsequent their
rapid correction modulate nitric oxide production dependent on NFAT5 in
microglia. Free Radic.
Biol. Med. 223: 458-472. doi:10.1016/j.freeradbiomed.2024.08.019
Hayashi, H., Mak, T.W.,
Tanaka, Y., Kubo, Y., Izumida, M., Urae, R. & Matsuyama, T. 2024. Development of a highly
sensitive platform for protein-protein interaction detection and regulation of
T cell function. Proc. Natl. Acad. Sci.
U.S.A. 121(33):
e2318190121. doi:10.1073/pnas.2318190121
Hiwase, A. D., Ovenden, C. D., Kaukas,
L. M., Finnis, M., Zhang, Z., O'Connor, S., Foo, N., Reddi,
B., Wells, A.J. & Ellis, D.Y. 2024. Can rotational thromboelastometry rapidly identify theragnostic targets in isolated traumatic brain injury? Emerg. Med. Australas.
doi:10.1111/1742-6723.14480
King, J., Friend, C., Zhang, D. & Carr, W. 2024. Comparative performance of three
eye-tracking devices in detection of mild traumatic brain injury in acute
versus chronic subject populations. Mil.
Med. 189(Supplement_3):
628-635. doi:10.1093/milmed/usae205
Li, Z., Yu, S., Li, L., Zhou, C., Wang, L., Tang,
S., Gu, N., Zhang, Z., Huang, Z., Chen, H.,
Tang, W., Wang, Y., Yang, X., Sun, X. & Yan, J. 2024. TREM2
alleviates white matter injury after traumatic brain injury in mice might be
mediated by regulation of DHCR24/LXR pathway in microglia. Clin. Transl. Med. 14(4):
e1665. doi:10.1002/ctm2.1665
Ma, X., Liao, Z., Tan, H., Wang, K., Feng, C.,
Xing, P., Zhang, X., Hua, J., Jiang, P., Peng, S., Lin, H., Liang, W. &
Gao, X. 2024. The association between cytomegalovirus infection and
neurodegenerative diseases: A prospective cohort using UK Biobank data. EClinicalMedicine 74: 102757. doi:10.1016/j.eclinm.2024.102757
Malarkey, M.E., Fu, A.J., Mannan, N., Shaw, O.M.,
Haight, T.J., Cota, M.R., Jahed, N.C., Werner, J.K.
& Brody, D.L. 2024. Internet-guided cognitive behavioral therapy for
insomnia among patients with traumatic brain injury: A randomized clinical
trial. JAMA Netw.
Open 7(7): e2420090.
doi:10.1001/jamanetworkopen.2024.20090
Metry, Y., McMullan, C., Upthegrove,
R., Belli, A., Gomes, R.S.M., Blanch, R.J. & Ahmed, Z. 2024. Understanding
how traumatic brain injury-related changes in fluid biomarkers affect quality
of life outcomes in veterans: A prospective observational trial protocol
(UNTANGLE). BMJ Open 14(8): e084818.
doi:10.1136/bmjopen-2024-084818
Minea, H., Singeap, A.M., Minea, M., Juncu, S., Muzica, C., Sfarti, C.V., Girleanu, I., Chiriac, S., Miftode, I.D., Stanciu, C.
& Trifan, A. 2024. The contribution
of genetic and epigenetic factors: An emerging concept in the assessment and
prognosis of inflammatory bowel diseases. Int.
J. Mol. Sci. 25(15): 8420.
doi:10.3390/ijms25158420
Oduro-Kwateng, E., Ali,
M., Kehinde, I.O., Zhang, Z. & Soliman, M.E.S. 2024. De novo rational
design of peptide-based protein-protein inhibitors (Pep-PPIs) approach by
mapping the interaction motifs of the PP interface and physicochemical
filtration: A case on p25-Cdk5-mediated neurodegenerative diseases. J. Cell Biochem.
125(9): e30633. doi:10.1002/jcb.30633
Ouyang, A., Chen, T., Feng, Y., Zou, J., Tu, S.,
Jiang, M., Sun, H. & Zhou, H. 2024. The hemagglutinin of influenza A virus
induces ferroptosis to facilitate viral replication. Adv. Sci. (Weinh) 11(39): e2404365.
doi:10.1002/advs.202404365
Rajkumar, R.P. 2024. Revisiting a hypothesis: The
neurovascular unit as a link between major depression and neurodegenerative
disorders. Front Cell Neurosci. 18: 1455606.
doi:10.3389/fncel.2024.1455606
Rapport, L.J., Kalpakjian,
C.Z., Sander, A.M., Lequerica, A.H., Bushnik, T., Quint, E.H. & Hanks, R.A. 2024. Menopause
and traumatic brain injury: A NIDILRR collaborative TBI model systems study. Arch. Phys. Med. Rehabil. doi:10.1016/j.apmr.2024.07.021
Sok, S.P.M., Pipkin, K., Popescu, N.I., Reidy, M., Li,
B., Van Remmen, H., Kinter,
M., Sun, X.H., Fan, Z. & Zhao, M. 2024. Gpx4 regulates invariant NKT cell
homeostasis and function by preventing lipid peroxidation and ferroptosis. J. Immunol. 213(7): 941-951.
doi:10.4049/jimmunol.2400246
Xu, Y., Xu, J., Zhu, Y., Mao, H., Li, J., Kong, X.,
Zhu, X. & Zhang, J. 2024. Investigating gut microbiota-blood and urine
metabolite correlations in early sepsis-induced acute kidney injury: Insights
from targeted KEGG analyses. Front Cell
Infect. Microbiol. 14: 1375874. doi:10.3389/fcimb.2024.1375874
Zhang, X., Shao, S., Song, N., Yang, B., Liu, F.,
Tong, Z., Wang, F. & Li, J. 2024. Integrated omics characterization reveals
reduced cancer indicators and elevated inflammatory factors after thermal
ablation in non-small cell lung cancer patients. Respir. Res. 25(1):
309. doi:10.1186/s12931-024-02917-9
*Pengarang untuk surat-menyurat; email: 350571178@qq.com
|
|||
|
